Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3631829..3632666 Replicon chromosome
Accession NZ_CP058748
Organism Escherichia coli strain STLIN_13

Toxin (Protein)


Gene name itaT Uniprot ID A0A1M0D5U3
Locus tag HZS99_RS17515 Protein ID WP_000227782.1
Coordinates 3632124..3632666 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag HZS99_RS17510 Protein ID WP_001353405.1
Coordinates 3631829..3632140 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS99_RS17485 (3626849) 3626849..3627796 + 948 WP_001239437.1 cytochrome o ubiquinol oxidase subunit II -
HZS99_RS17490 (3627818) 3627818..3629809 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HZS99_RS17495 (3629799) 3629799..3630413 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HZS99_RS17500 (3630413) 3630413..3630742 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HZS99_RS17505 (3630754) 3630754..3631644 + 891 WP_000971327.1 heme o synthase -
HZS99_RS17510 (3631829) 3631829..3632140 + 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
HZS99_RS17515 (3632124) 3632124..3632666 + 543 WP_000227782.1 GNAT family N-acetyltransferase Toxin
HZS99_RS17520 (3632722) 3632722..3633566 - 845 Protein_3442 tetratricopeptide repeat protein -
HZS99_RS17525 (3633973) 3633973..3635337 + 1365 WP_001000978.1 MFS transporter -
HZS99_RS17530 (3635465) 3635465..3635956 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HZS99_RS17535 (3636124) 3636124..3637035 + 912 WP_000705841.1 2-dehydropantoate 2-reductase -
HZS99_RS17540 (3636998) 3636998..3637588 + 591 WP_001276320.1 protein deglycase YajL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19957.31 Da        Isoelectric Point: 9.1763

>T167353 WP_000227782.1 NZ_CP058748:3632124-3632666 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T167353 NZ_CP075116:c4055034-4054750 [Salmonella enterica subsp. enterica serovar Stanleyville]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT167353 WP_001353405.1 NZ_CP058748:3631829-3632140 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT167353 NZ_CP075116:c4055266-4055024 [Salmonella enterica subsp. enterica serovar Stanleyville]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0D5U3

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References