Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3597739..3598357 Replicon chromosome
Accession NZ_CP058748
Organism Escherichia coli strain STLIN_13

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HZS99_RS17345 Protein ID WP_001291435.1
Coordinates 3598139..3598357 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HZS99_RS17340 Protein ID WP_000344800.1
Coordinates 3597739..3598113 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS99_RS17330 (3592828) 3592828..3594021 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HZS99_RS17335 (3594044) 3594044..3597193 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HZS99_RS17340 (3597739) 3597739..3598113 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HZS99_RS17345 (3598139) 3598139..3598357 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HZS99_RS17350 (3598529) 3598529..3599080 + 552 WP_000102574.1 maltose O-acetyltransferase -
HZS99_RS17355 (3599196) 3599196..3599666 + 471 WP_000136192.1 YlaC family protein -
HZS99_RS17360 (3599830) 3599830..3601380 + 1551 WP_001361689.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HZS99_RS17365 (3601422) 3601422..3601775 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HZS99_RS17375 (3602154) 3602154..3602465 + 312 WP_000409911.1 MGMT family protein -
HZS99_RS17380 (3602496) 3602496..3603068 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T167352 WP_001291435.1 NZ_CP058748:3598139-3598357 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T167352 NZ_CP075116:c3961345-3961058 [Salmonella enterica subsp. enterica serovar Stanleyville]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT167352 WP_000344800.1 NZ_CP058748:3597739-3598113 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT167352 NZ_CP075116:c3961071-3960769 [Salmonella enterica subsp. enterica serovar Stanleyville]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References