Detailed information of TA system TA167340

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1983241..1984025
• Replicon: chromosome
• Accession: NZ_CP058748
• Organism: Escherichia coli strain STLIN_13

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: HZS99_RS09440
• Protein ID: WP_000613626.1
• Coordinates: 1983241..1983735 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: HZS99_RS09445
• Protein ID: WP_001110447.1
• Coordinates: 1983732..1984025 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS99_RS09420 (1978434)	1978434..1979531	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
HZS99_RS09425 (1979531)	1979531..1980472	+	942	WP_001305885.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
HZS99_RS09430 (1980538)	1980538..1982181	+	1644	WP_061092652.1	flagellar hook-associated protein FlgK	-
HZS99_RS09435 (1982193)	1982193..1983146	+	954	WP_001212762.1	flagellar hook-associated protein FlgL	-
HZS99_RS09440 (1983241)	1983241..1983735	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
HZS99_RS09445 (1983732)	1983732..1984025	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
HZS99_RS09450 (1984158)	1984158..1987343	-	3186	WP_061092653.1	ribonuclease E	-
HZS99_RS09455 (1987916)	1987916..1988875	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T167340 WP_000613626.1 NZ_CP058748:c1983735-1983241 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T167340 NZ_CP075115:c4380774-4380463 [Salmonella enterica subsp. enterica serovar Eastbourne]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCATCGTAGTCAACGGTATGAGGTTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT167340 WP_001110447.1 NZ_CP058748:c1984025-1983732 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT167340 NZ_CP075115:c4380462-4380172 [Salmonella enterica subsp. enterica serovar Eastbourne]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACTTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6