Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 933882..934536 Replicon chromosome
Accession NZ_CP058748
Organism Escherichia coli strain STLIN_13

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HZS99_RS04580 Protein ID WP_000244781.1
Coordinates 934129..934536 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZS99_RS04575 Protein ID WP_000354046.1
Coordinates 933882..934148 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS99_RS04555 (929970) 929970..931403 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
HZS99_RS04560 (931448) 931448..931759 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
HZS99_RS04565 (931923) 931923..932582 + 660 WP_000250269.1 hemolysin III family protein -
HZS99_RS04570 (932659) 932659..933639 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
HZS99_RS04575 (933882) 933882..934148 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZS99_RS04580 (934129) 934129..934536 + 408 WP_000244781.1 protein YgfX Toxin
HZS99_RS04585 (934576) 934576..935097 - 522 WP_001521233.1 flavodoxin FldB -
HZS99_RS04590 (935209) 935209..936105 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HZS99_RS04595 (936130) 936130..936840 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZS99_RS04600 (936846) 936846..938579 + 1734 WP_063117505.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T167338 WP_000244781.1 NZ_CP058748:934129-934536 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T167338 NZ_CP075115:c3940913-3940629 [Salmonella enterica subsp. enterica serovar Eastbourne]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTGGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT167338 WP_000354046.1 NZ_CP058748:933882-934148 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT167338 NZ_CP075115:c3941145-3940903 [Salmonella enterica subsp. enterica serovar Eastbourne]
ATGGCCACGCTGAACGTCCGTCTGGATGAAAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAGGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References