Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 350470..351101 Replicon chromosome
Accession NZ_CP058748
Organism Escherichia coli strain STLIN_13

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag HZS99_RS01595 Protein ID WP_001260301.1
Coordinates 350470..350745 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HZS99_RS01600 Protein ID WP_000593555.1
Coordinates 350742..351101 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS99_RS01580 (345474) 345474..346541 + 1068 WP_000361497.1 HlyD family efflux transporter periplasmic adaptor subunit -
HZS99_RS01585 (346538) 346538..349273 + 2736 WP_061092822.1 ribosome-associated ATPase/putative transporter RbbA -
HZS99_RS01590 (349273) 349273..350397 + 1125 WP_001314210.1 ABC transporter permease -
HZS99_RS01595 (350470) 350470..350745 + 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
HZS99_RS01600 (350742) 350742..351101 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HZS99_RS01605 (351221) 351221..351622 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HZS99_RS01610 (351627) 351627..352433 - 807 WP_061092823.1 nickel import ATP-binding protein NikE -
HZS99_RS01615 (352430) 352430..353194 - 765 WP_001136211.1 nickel import ATP-binding protein NikD -
HZS99_RS01620 (353194) 353194..354027 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HZS99_RS01625 (354024) 354024..354968 - 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 343486..351622 8136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T167334 WP_001260301.1 NZ_CP058748:350470-350745 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T167334 NZ_CP075115:1925138-1925241 [Salmonella enterica subsp. enterica serovar Eastbourne]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT167334 WP_000593555.1 NZ_CP058748:350742-351101 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT167334 NZ_CP075115:c1925243-1925100 [Salmonella enterica subsp. enterica serovar Eastbourne]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References