Detailed information of TA system TA167332

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 70187..70985
• Replicon: chromosome
• Accession: NZ_CP058748
• Organism: Escherichia coli strain STLIN_13

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HZS99_RS00305
• Protein ID: WP_061092894.1
• Coordinates: 70187..70564 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HZS99_RS00310
• Protein ID: WP_061092893.1
• Coordinates: 70611..70985 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS99_RS00275 (65550)	65550..66473	-	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-
HZS99_RS00280 (66584)	66584..67768	-	1185	WP_001172864.1	sugar efflux transporter	-
HZS99_RS00285 (68167)	68167..68328	-	162	Protein_56	RhuM family protein	-
HZS99_RS00290 (68566)	68566..69408	-	843	WP_061092922.1	DUF4942 domain-containing protein	-
HZS99_RS00295 (69493)	69493..69690	-	198	WP_012908799.1	DUF957 domain-containing protein	-
HZS99_RS00300 (69702)	69702..70190	-	489	WP_061092895.1	DUF5983 family protein	-
HZS99_RS00305 (70187)	70187..70564	-	378	WP_061092894.1	TA system toxin CbtA family protein	Toxin
HZS99_RS00310 (70611)	70611..70985	-	375	WP_061092893.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HZS99_RS00315 (71148)	71148..71369	-	222	WP_061092892.1	DUF987 domain-containing protein	-
HZS99_RS00320 (71432)	71432..71908	-	477	WP_001186188.1	RadC family protein	-
HZS99_RS00325 (71923)	71923..72402	-	480	WP_000706978.1	antirestriction protein	-
HZS99_RS00330 (72668)	72668..73486	-	819	WP_001175165.1	DUF932 domain-containing protein	-
HZS99_RS00335 (73576)	73576..73809	-	234	WP_001278293.1	DUF905 family protein	-
HZS99_RS00340 (73815)	73815..74492	-	678	WP_001097302.1	hypothetical protein	-
HZS99_RS00345 (74640)	74640..75320	-	681	WP_001282921.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	58841..84548	25707

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.15 Da
• Isoelectric Point: 7.8045

>T167332 WP_061092894.1 NZ_CP058748:c70564-70187 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALIRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T167332 NZ_CP075115:312880-313248 [Salmonella enterica subsp. enterica serovar Eastbourne]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
TATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13752.53 Da
• Isoelectric Point: 6.5924

>AT167332 WP_061092893.1 NZ_CP058748:c70985-70611 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNHLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT167332 NZ_CP075115:312662-312883 [Salmonella enterica subsp. enterica serovar Eastbourne]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGCGGGCCCCGTCAC
CATCACGCGTCGTGGGCATAAGCCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA