Detailed information of TA system TA167331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 39817..40553
• Replicon: plasmid unnamed1
• Accession: NZ_CP058744
• Organism: Klebsiella pneumoniae strain STLIN_10

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: HZS96_RS26335
• Protein ID: WP_004187044.1
• Coordinates: 40071..40553 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HZS96_RS26330
• Protein ID: WP_003026799.1
• Coordinates: 39817..40083 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS96_RS26310 (HZS96_26210)	34966..35946	+	981	Protein_36	IS5-like element ISKpn26 family transposase	-
HZS96_RS26315 (HZS96_26215)	36004..36249	+	246	WP_032238678.1	hypothetical protein	-
HZS96_RS26320 (HZS96_26220)	36218..38803	+	2586	WP_004187040.1	EAL domain-containing protein	-
HZS96_RS26325 (HZS96_26225)	38962..39666	-	705	WP_060579418.1	IS6-like element IS26 family transposase	-
HZS96_RS26330 (HZS96_26230)	39817..40083	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HZS96_RS26335 (HZS96_26235)	40071..40553	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
HZS96_RS26340 (HZS96_26240)	40764..42110	+	1347	WP_240726744.1	ISNCY family transposase	-
HZS96_RS26345 (HZS96_26245)	42270..42974	+	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
HZS96_RS26350 (HZS96_26250)	43208..43318	-	111	Protein_44	IS3 family transposase	-
HZS96_RS26355 (HZS96_26260)	43593..44529	+	937	Protein_45	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..130177	130177
	-	inside	IScluster/Tn	-	-	34966..44550	9584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T167331 WP_004187044.1 NZ_CP058744:40071-40553 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T167331 NZ_CP075115:208085-208570 [Salmonella enterica subsp. enterica serovar Eastbourne]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACTTTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT167331 WP_003026799.1 NZ_CP058744:39817-40083 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT167331 NZ_CP075115:207810-208097 [Salmonella enterica subsp. enterica serovar Eastbourne]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66