Detailed information of TA system TA167330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 26400..26922
• Replicon: plasmid unnamed1
• Accession: NZ_CP058744
• Organism: Klebsiella pneumoniae strain STLIN_10

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: HZS96_RS26240
• Protein ID: WP_004187019.1
• Coordinates: 26400..26684 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: HZS96_RS26245
• Protein ID: WP_004187025.1
• Coordinates: 26674..26922 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS96_RS26220 (HZS96_26125)	21619..22083	-	465	WP_004187010.1	conjugative transfer protein MobI(A/C)	-
HZS96_RS26225 (HZS96_26130)	23626..24039	+	414	WP_004187013.1	hypothetical protein	-
HZS96_RS26230	24696..24947	+	252	Protein_20	conjugal transfer protein TraH	-
HZS96_RS26235 (HZS96_26140)	25029..26384	+	1356	WP_240726561.1	ISNCY family transposase	-
HZS96_RS26240 (HZS96_26145)	26400..26684	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZS96_RS26245 (HZS96_26150)	26674..26922	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
HZS96_RS26250 (HZS96_26155)	27212..27636	-	425	Protein_24	IS1 family transposase	-
HZS96_RS26255 (HZS96_26160)	27824..27940	-	117	Protein_25	transposase domain-containing protein	-
HZS96_RS26260 (HZS96_26165)	27885..28148	-	264	WP_004187027.1	transposase	-
HZS96_RS26265 (HZS96_26170)	28163..28426	+	264	WP_004118208.1	hypothetical protein	-
HZS96_RS26270 (HZS96_26175)	28670..28951	+	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
HZS96_RS26275 (HZS96_26180)	28986..29555	+	570	WP_004152117.1	small heat shock protein sHSP20	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..130177	130177
	-	flank	IS/Tn	-	-	27176..27442	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T167330 WP_004187019.1 NZ_CP058744:c26684-26400 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T167330 NZ_CP075114:73351-73632 [Salmonella enterica subsp. enterica serovar Dublin]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT167330 WP_004187025.1 NZ_CP058744:c26922-26674 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT167330 NZ_CP075114:73110-73361 [Salmonella enterica subsp. enterica serovar Dublin]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4