Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5247790..5248415 Replicon chromosome
Accession NZ_CP058743
Organism Klebsiella pneumoniae strain STLIN_10

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag HZS96_RS25765 Protein ID WP_004187928.1
Coordinates 5247790..5248173 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HZS96_RS25770 Protein ID WP_004150355.1
Coordinates 5248173..5248415 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS96_RS25750 (5245156) 5245156..5246058 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HZS96_RS25755 (5246055) 5246055..5246690 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HZS96_RS25760 (5246687) 5246687..5247616 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HZS96_RS25765 (5247790) 5247790..5248173 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
HZS96_RS25770 (5248173) 5248173..5248415 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HZS96_RS25775 (5248609) 5248609..5249526 + 918 WP_004187929.1 alpha/beta hydrolase -
HZS96_RS25780 (5249540) 5249540..5250481 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HZS96_RS25785 (5250526) 5250526..5250963 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HZS96_RS25790 (5250960) 5250960..5251820 - 861 WP_004146232.1 virulence factor BrkB family protein -
HZS96_RS25795 (5251814) 5251814..5252413 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T167329 WP_004187928.1 NZ_CP058743:c5248173-5247790 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T167329 NZ_CP075114:45663-45968 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT167329 WP_004150355.1 NZ_CP058743:c5248415-5248173 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT167329 NZ_CP075114:45443-45661 [Salmonella enterica subsp. enterica serovar Dublin]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References