Detailed information of TA system TA167295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 66782..67304
• Replicon: plasmid pS171-1.2
• Accession: NZ_CP058727
• Organism: Escherichia coli strain S171-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0QMR1
• Locus tag: HZS87_RS23495
• Protein ID: WP_000220561.1
• Coordinates: 67023..67304 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: HZS87_RS23490
• Protein ID: WP_000121743.1
• Coordinates: 66782..67033 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS87_RS23470 (62905)	62905..63258	-	354	WP_024129958.1	DNA distortion polypeptide 3	-
HZS87_RS23475 (63395)	63395..63841	-	447	WP_001074384.1	hypothetical protein	-
HZS87_RS23480 (63845)	63845..64687	-	843	WP_063116919.1	replication initiation protein	-
HZS87_RS23485 (64708)	64708..65547	-	840	WP_024129959.1	replication initiation protein	-
HZS87_RS23490 (66782)	66782..67033	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
HZS87_RS23495 (67023)	67023..67304	+	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZS87_RS23500 (67543)	67543..67635	-	93	Protein_75	IS6 family transposase	-
HZS87_RS23505 (67801)	67801..69000	-	1200	WP_000948429.1	IS91 family transposase	-
HZS87_RS23510 (69010)	69010..69198	-	189	WP_000957857.1	hypothetical protein	-
HZS87_RS23515 (69318)	69318..70748	+	1431	Protein_78	IS4-like element IS50R family transposase	-
HZS87_RS23520 (70777)	70777..71571	+	795	WP_000572405.1	aminoglycoside O-phosphotransferase APH(3')-IIa	-
HZS87_RS23525 (71592)	71592..71828	+	237	Protein_80	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / aph(3')-IIa / tet(A) / ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / dfrA14 / blaCTX-M-65 / qepA1	-	1..91934	91934

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11023.86 Da
• Isoelectric Point: 10.5938

>T167295 WP_000220561.1 NZ_CP058727:67023-67304 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Nucleotide

• Length: 282 bp

>T167295 NZ_CP075110:c4242484-4241993 [Salmonella enterica]
ATGATCTCCGCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAGCGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAAGTGGAGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT167295 WP_000121743.1 NZ_CP058727:66782-67033 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT167295 NZ_CP075110:c4242774-4242481 [Salmonella enterica]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCCGCCCGCGCTGCCGCAGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCACAGGCTTATCAGGAATTTCTCGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A3S5I302

Antitoxin

• AlphaFold DB: A0A3S5I301