Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 18086..18608 Replicon plasmid pS171-1.2
Accession NZ_CP058727
Organism Escherichia coli strain S171-1

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag HZS87_RS23215 Protein ID WP_000220561.1
Coordinates 18327..18608 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag HZS87_RS23210 Protein ID WP_000121743.1
Coordinates 18086..18337 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS87_RS23190 (14209) 14209..14562 - 354 WP_024129958.1 DNA distortion polypeptide 3 -
HZS87_RS23195 (14699) 14699..15145 - 447 WP_001074384.1 hypothetical protein -
HZS87_RS23200 (15149) 15149..15991 - 843 WP_063116919.1 replication initiation protein -
HZS87_RS23205 (16012) 16012..16851 - 840 WP_024129959.1 replication initiation protein -
HZS87_RS23210 (18086) 18086..18337 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
HZS87_RS23215 (18327) 18327..18608 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HZS87_RS23220 (18847) 18847..18939 - 93 Protein_19 IS6 family transposase -
HZS87_RS23225 (19105) 19105..20304 - 1200 WP_000948429.1 IS91 family transposase -
HZS87_RS23230 (20314) 20314..20502 - 189 WP_000957857.1 hypothetical protein -
HZS87_RS23235 (20622) 20622..22052 + 1431 Protein_22 IS4-like element IS50R family transposase -
HZS87_RS23240 (22081) 22081..22875 + 795 WP_000572405.1 aminoglycoside O-phosphotransferase APH(3')-IIa -
HZS87_RS23245 (22896) 22896..23132 + 237 Protein_24 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / aph(3')-IIa / tet(A) / ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / dfrA14 / blaCTX-M-65 / qepA1 - 1..91934 91934


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T167294 WP_000220561.1 NZ_CP058727:18327-18608 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T167294 NZ_CP075110:c4104381-4104097 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCTGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT167294 WP_000121743.1 NZ_CP058727:18086-18337 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT167294 NZ_CP075110:c4104613-4104371 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAATGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References