Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 103364..104007 Replicon plasmid pS171-1.1
Accession NZ_CP058726
Organism Escherichia coli strain S171-1

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag HZS87_RS22840 Protein ID WP_001044768.1
Coordinates 103591..104007 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag HZS87_RS22835 Protein ID WP_001261287.1
Coordinates 103364..103594 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS87_RS22820 (99475) 99475..100563 + 1089 WP_000952231.1 hypothetical protein -
HZS87_RS22825 (100565) 100565..101434 + 870 WP_000253407.1 hypothetical protein -
HZS87_RS22830 (101491) 101491..103056 - 1566 WP_000741348.1 AAA family ATPase -
HZS87_RS22835 (103364) 103364..103594 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZS87_RS22840 (103591) 103591..104007 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
HZS87_RS22845 (104169) 104169..106307 - 2139 WP_000350635.1 AAA family ATPase -
HZS87_RS22850 (106661) 106661..106918 + 258 WP_000343085.1 hypothetical protein -
HZS87_RS22855 (106918) 106918..107508 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / dfrA14 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1A / aac(3)-IId iroB / iroC / iroD / iroE / iroN 1..152180 152180


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T167293 WP_001044768.1 NZ_CP058726:103591-104007 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T167293 NZ_CP075110:c4063788-4063480 [Salmonella enterica]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGGTTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT167293 WP_001261287.1 NZ_CP058726:103364-103594 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT167293 NZ_CP075110:c4064030-4063791 [Salmonella enterica]
ATGTCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCTGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTGCTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References