Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 19025..19547 Replicon plasmid pM159-1.2
Accession NZ_CP058721
Organism Escherichia coli strain M159-1

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag HZS86_RS22680 Protein ID WP_000220561.1
Coordinates 19266..19547 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag HZS86_RS22675 Protein ID WP_000121743.1
Coordinates 19025..19276 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS86_RS22645 (14082) 14082..14435 - 354 WP_024129958.1 DNA distortion polypeptide 3 -
HZS86_RS22650 (14572) 14572..15018 - 447 WP_001074384.1 hypothetical protein -
HZS86_RS22655 (15022) 15022..15864 - 843 WP_063116919.1 replication initiation protein -
HZS86_RS22660 (15885) 15885..16370 - 486 WP_239802617.1 replication initiation protein -
HZS86_RS22665 (16447) 16447..17415 - 969 WP_000654804.1 IS5-like element IS903B family transposase -
HZS86_RS22670 (17437) 17437..17790 - 354 WP_239802618.1 replication initiation protein -
HZS86_RS22675 (19025) 19025..19276 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
HZS86_RS22680 (19266) 19266..19547 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HZS86_RS22685 (19786) 19786..19878 - 93 Protein_25 IS6 family transposase -
HZS86_RS22690 (20044) 20044..20553 - 510 Protein_26 transposase -
HZS86_RS22695 (20608) 20608..21312 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
HZS86_RS22700 (21559) 21559..22032 - 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
HZS86_RS22705 (22060) 22060..22898 - 839 Protein_29 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1 -
HZS86_RS22710 (22915) 22915..23715 - 801 WP_000846390.1 oxacillin-hydrolyzing class D beta-lactamase OXA-10 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid fosA3 / blaTEM-1B / dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / tet(A) / aph(3')-IIa / blaCTX-M-65 / qepA1 - 1..90772 90772
- inside IScluster/Tn dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / tet(A) / aph(3')-IIa / blaCTX-M-65 / qepA1 - 16447..56353 39906


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T167266 WP_000220561.1 NZ_CP058721:19266-19547 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T167266 NZ_CP075108:1944657-1944760 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT167266 WP_000121743.1 NZ_CP058721:19025-19276 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT167266 NZ_CP075108:c1944762-1944617 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References