Detailed information of TA system TA167255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3295415..3296053
• Replicon: chromosome
• Accession: NZ_CP058719
• Organism: Escherichia coli strain M159-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HZS86_RS15995
• Protein ID: WP_000813794.1
• Coordinates: 3295415..3295591 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HZS86_RS16000
• Protein ID: WP_001270286.1
• Coordinates: 3295637..3296053 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS86_RS15975 (3291034)	3291034..3292209	-	1176	WP_001236251.1	BenE family transporter YdcO	-
HZS86_RS15980 (3292301)	3292301..3292837	+	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
HZS86_RS15985 (3292910)	3292910..3294871	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HZS86_RS15990 (3294963)	3294963..3295193	-	231	WP_000494244.1	YncJ family protein	-
HZS86_RS15995 (3295415)	3295415..3295591	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HZS86_RS16000 (3295637)	3295637..3296053	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HZS86_RS16005 (3296132)	3296132..3297538	+	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
HZS86_RS16010 (3297783)	3297783..3298928	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
HZS86_RS16015 (3298946)	3298946..3299959	+	1014	WP_072643648.1	ABC transporter ATP-binding protein	-
HZS86_RS16020 (3299960)	3299960..3300901	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3289729..3290994	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T167255 WP_000813794.1 NZ_CP058719:3295415-3295591 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T167255 NZ_CP075107:c962251-961724 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGCCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTACTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT167255 WP_001270286.1 NZ_CP058719:3295637-3296053 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT167255 NZ_CP075107:c962538-962248 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• PDB: 6HPB