Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1780202..1780778 Replicon chromosome
Accession NZ_CP058719
Organism Escherichia coli strain M159-1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HZS86_RS08680 Protein ID WP_001297643.1
Coordinates 1780202..1780489 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HZS86_RS08685 Protein ID WP_000063156.1
Coordinates 1780476..1780778 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS86_RS08660 (1776455) 1776455..1777735 - 1281 WP_001037400.1 DUF445 domain-containing protein -
HZS86_RS08665 (1777776) 1777776..1779008 - 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
HZS86_RS08670 (1779154) 1779154..1779249 - 96 Protein_1690 dienelactone hydrolase family protein -
HZS86_RS08675 (1779306) 1779306..1780003 + 698 Protein_1691 IS1 family transposase -
HZS86_RS08680 (1780202) 1780202..1780489 + 288 WP_001297643.1 BrnT family toxin Toxin
HZS86_RS08685 (1780476) 1780476..1780778 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HZS86_RS08690 (1780812) 1780812..1781768 - 957 WP_001297640.1 GTPase -
HZS86_RS08695 (1781779) 1781779..1781982 - 204 WP_000467859.1 YbdD/YjiX family protein -
HZS86_RS08700 (1782032) 1782032..1784182 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T167251 WP_001297643.1 NZ_CP058719:1780202-1780489 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T167251 NZ_CP075106:c4614020-4613646 [Salmonella enterica]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAGAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGCGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAGCGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCTCAAGTCCATCGTTGTGAGCTTGTAACGCG
TAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACACCGTATCACTTGTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT167251 WP_000063156.1 NZ_CP058719:1780476-1780778 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167251 NZ_CP075106:c4614262-4614020 [Salmonella enterica]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTTGATTTATCAGACGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTGCCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References