Detailed information of TA system TA167250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1596399..1596997
• Replicon: chromosome
• Accession: NZ_CP058719
• Organism: Escherichia coli strain M159-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: HZS86_RS07725
• Protein ID: WP_053291281.1
• Coordinates: 1596620..1596997 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: HZS86_RS07720
• Protein ID: WP_001603498.1
• Coordinates: 1596399..1596620 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS86_RS07695 (1592374)	1592374..1592568	+	195	WP_015060486.1	hypothetical protein	-
HZS86_RS07700 (1592760)	1592760..1593011	-	252	Protein_1500	IS200/IS605 family transposase	-
HZS86_RS07705 (1593067)	1593067..1593764	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
HZS86_RS07710 (1593780)	1593780..1595183	+	1404	Protein_1502	fimbria/pilus outer membrane usher protein	-
HZS86_RS07715 (1595195)	1595195..1596214	+	1020	WP_095784823.1	fimbrial protein	-
HZS86_RS07720 (1596399)	1596399..1596620	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HZS86_RS07725 (1596620)	1596620..1596997	+	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HZS86_RS07730 (1597107)	1597107..1597322	-	216	Protein_1506	transposase	-
HZS86_RS07735 (1597508)	1597508..1598770	-	1263	WP_096263470.1	integrase arm-type DNA-binding domain-containing protein	-
HZS86_RS07745 (1599150)	1599150..1599725	-	576	WP_001188520.1	transcriptional regulator	-
HZS86_RS07750 (1599762)	1599762..1601459	-	1698	WP_072643567.1	protein-disulfide reductase DsbD	-
HZS86_RS07755 (1601435)	1601435..1601773	-	339	WP_000883400.1	divalent cation tolerance protein CutA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	htpB	1575745..1615993	40248
	-	inside	IScluster/Tn	-	-	1581130..1597322	16192

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T167250 WP_053291281.1 NZ_CP058719:1596620-1596997 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T167250 NZ_CP075106:c4301632-4301141 [Salmonella enterica]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAAGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT167250 WP_001603498.1 NZ_CP058719:1596399-1596620 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT167250 NZ_CP075106:c4301922-4301629 [Salmonella enterica]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGTGAAACTCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCAGAAGAAGCGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAATTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Antitoxin

• AlphaFold DB: L4J1C0