Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 82007..82554 Replicon plasmid pM172-1.1
Accession NZ_CP058715
Organism Escherichia coli strain M172-1

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag HYI45_RS24420 Protein ID WP_001384452.1
Coordinates 82276..82554 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A723YP93
Locus tag HYI45_RS24415 Protein ID WP_021559054.1
Coordinates 82007..82276 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYI45_RS24380 (77126) 77126..77686 + 561 WP_094891561.1 fertility inhibition protein FinO -
HYI45_RS24385 (77820) 77820..78026 + 207 WP_072656937.1 ANR family transcriptional regulator -
HYI45_RS24390 (78416) 78416..79015 + 600 WP_072656938.1 PIN domain-containing protein -
HYI45_RS24395 (79674) 79674..79928 + 255 WP_000083830.1 replication regulatory protein RepA -
HYI45_RS24400 (80167) 80167..80241 + 75 WP_001365705.1 RepA leader peptide Tap -
HYI45_RS24405 (80234) 80234..81091 + 858 WP_000130962.1 incFII family plasmid replication initiator RepA -
HYI45_RS24410 (81794) 81794..81952 + 159 WP_000841008.1 hypothetical protein -
HYI45_RS24415 (82007) 82007..82276 + 270 WP_021559054.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
HYI45_RS24420 (82276) 82276..82554 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
HYI45_RS24425 (82877) 82877..83455 + 579 WP_231235770.1 hypothetical protein -
HYI45_RS24430 (83794) 83794..83949 - 156 Protein_104 IS91 family transposase -
HYI45_RS24435 (83966) 83966..84202 - 237 WP_034169385.1 hypothetical protein -
HYI45_RS24445 (85751) 85751..86812 + 1062 WP_034169386.1 DUF262 domain-containing protein -
HYI45_RS24450 (86816) 86816..87451 + 636 WP_034169387.1 MAE_28990/MAE_18760 family HEPN-like nuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..126040 126040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T167235 WP_001384452.1 NZ_CP058715:82276-82554 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T167235 NZ_CP075064:100274-100423 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10222.60 Da        Isoelectric Point: 8.8735

>AT167235 WP_021559054.1 NZ_CP058715:82007-82276 [Escherichia coli]
MAQINMSLRIDAELKDAFMAAAKSMDRNASQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT167235 NZ_CP075064:c100229-100170 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A723YP93

References