Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 14964..15511 Replicon plasmid pM172-1.1
Accession NZ_CP058715
Organism Escherichia coli strain M172-1

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag HYI45_RS23980 Protein ID WP_001384452.1
Coordinates 15233..15511 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A723YP93
Locus tag HYI45_RS23975 Protein ID WP_021559054.1
Coordinates 14964..15233 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYI45_RS23940 (10083) 10083..10643 + 561 WP_094891561.1 fertility inhibition protein FinO -
HYI45_RS23945 (10777) 10777..10983 + 207 WP_072656937.1 ANR family transcriptional regulator -
HYI45_RS23950 (11373) 11373..11972 + 600 WP_072656938.1 PIN domain-containing protein -
HYI45_RS23955 (12631) 12631..12885 + 255 WP_000083830.1 replication regulatory protein RepA -
HYI45_RS23960 (13124) 13124..13198 + 75 WP_001365705.1 RepA leader peptide Tap -
HYI45_RS23965 (13191) 13191..14048 + 858 WP_000130962.1 incFII family plasmid replication initiator RepA -
HYI45_RS23970 (14751) 14751..14909 + 159 WP_000841008.1 hypothetical protein -
HYI45_RS23975 (14964) 14964..15233 + 270 WP_021559054.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
HYI45_RS23980 (15233) 15233..15511 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
HYI45_RS23985 (15834) 15834..16412 + 579 WP_231235770.1 hypothetical protein -
HYI45_RS23990 (16751) 16751..16906 - 156 Protein_16 IS91 family transposase -
HYI45_RS23995 (16923) 16923..17159 - 237 WP_034169385.1 hypothetical protein -
HYI45_RS24005 (18708) 18708..19769 + 1062 WP_034169386.1 DUF262 domain-containing protein -
HYI45_RS24010 (19773) 19773..20408 + 636 WP_034169387.1 MAE_28990/MAE_18760 family HEPN-like nuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..126040 126040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T167232 WP_001384452.1 NZ_CP058715:15233-15511 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T167232 NZ_CP075063:c76807-76658 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10222.60 Da        Isoelectric Point: 8.8735

>AT167232 WP_021559054.1 NZ_CP058715:14964-15233 [Escherichia coli]
MAQINMSLRIDAELKDAFMAAAKSMDRNASQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT167232 NZ_CP075063:76851-76912 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A723YP93

References