Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3979561..3980345 Replicon chromosome
Accession NZ_CP058714
Organism Escherichia coli strain M172-1

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag HYI45_RS18925 Protein ID WP_000613626.1
Coordinates 3979561..3980055 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B1LI61
Locus tag HYI45_RS18930 Protein ID WP_001110446.1
Coordinates 3980052..3980345 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYI45_RS18905 (3974754) 3974754..3975851 + 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -
HYI45_RS18910 (3975851) 3975851..3976792 + 942 WP_012311956.1 flagellar assembly peptidoglycan hydrolase FlgJ -
HYI45_RS18915 (3976858) 3976858..3978501 + 1644 WP_000096448.1 flagellar hook-associated protein FlgK -
HYI45_RS18920 (3978513) 3978513..3979466 + 954 WP_001212763.1 flagellar hook-associated protein FlgL -
HYI45_RS18925 (3979561) 3979561..3980055 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
HYI45_RS18930 (3980052) 3980052..3980345 - 294 WP_001110446.1 DUF1778 domain-containing protein Antitoxin
HYI45_RS18935 (3980479) 3980479..3983652 - 3174 WP_023141578.1 ribonuclease E -
HYI45_RS18940 (3984225) 3984225..3985184 + 960 WP_000846337.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T167218 WP_000613626.1 NZ_CP058714:c3980055-3979561 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T167218 NZ_CP075062:1904006-1904257 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11079.67 Da        Isoelectric Point: 10.5423

>AT167218 WP_001110446.1 NZ_CP058714:c3980345-3980052 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT167218 NZ_CP075062:1904254-1904508 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G775

References