Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1442192..1442689 Replicon chromosome
Accession NZ_CP058714
Organism Escherichia coli strain M172-1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HYI45_RS06980 Protein ID WP_001173343.1
Coordinates 1442192..1442365 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HYI45_RS06985 Protein ID WP_000398619.1
Coordinates 1442393..1442689 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYI45_RS06970 (1438862) 1438862..1440319 + 1458 WP_000856843.1 dipeptide/tripeptide permease DtpC -
HYI45_RS06975 (1440556) 1440556..1442073 + 1518 WP_000003799.1 lysine--tRNA ligase -
HYI45_RS06980 (1442192) 1442192..1442365 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HYI45_RS06985 (1442393) 1442393..1442689 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HYI45_RS06990 (1442918) 1442918..1443190 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
HYI45_RS06995 (1443202) 1443202..1443432 - 231 WP_000371713.1 4Fe-4S mono-cluster protein YjdI -
HYI45_RS07000 (1443613) 1443613..1445244 + 1632 WP_001216471.1 sensor histidine kinase -
HYI45_RS07005 (1445241) 1445241..1445960 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T167213 WP_001173343.1 NZ_CP058714:c1442365-1442192 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T167213 NZ_CP075062:c775261-774884 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGCCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATACGGTGAACTTTCTCGTTGAAAAATACGCACTGATACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAACATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT167213 WP_000398619.1 NZ_CP058714:c1442689-1442393 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT167213 NZ_CP075062:c775719-775351 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References