Detailed information of TA system TA167212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 1336491..1337086
• Replicon: chromosome
• Accession: NZ_CP058714
• Organism: Escherichia coli strain M172-1

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: HYI45_RS06445
• Protein ID: WP_000239581.1
• Coordinates: 1336491..1336841 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: HYI45_RS06450
• Protein ID: WP_001223213.1
• Coordinates: 1336835..1337086 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYI45_RS06425 (1331945)	1331945..1332967	-	1023	WP_012311695.1	ABC transporter permease	-
HYI45_RS06430 (1332981)	1332981..1334483	-	1503	WP_001095664.1	sugar ABC transporter ATP-binding protein	-
HYI45_RS06435 (1334616)	1334616..1335572	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
HYI45_RS06440 (1335882)	1335882..1336412	+	531	WP_000055075.1	inorganic diphosphatase	-
HYI45_RS06445 (1336491)	1336491..1336841	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
HYI45_RS06450 (1336835)	1336835..1337086	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
HYI45_RS06455 (1337298)	1337298..1337639	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
HYI45_RS06460 (1337642)	1337642..1341421	-	3780	WP_000060872.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T167212 WP_000239581.1 NZ_CP058714:c1336841-1336491 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T167212 NZ_CP075062:c588366-587902 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT167212 WP_001223213.1 NZ_CP058714:c1337086-1336835 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT167212 NZ_CP075062:c588701-588366 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7