Detailed information of TA system TA167210

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 784011..784717
• Replicon: chromosome
• Accession: NZ_CP058714
• Organism: Escherichia coli strain M172-1

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: V0SXW1
• Locus tag: HYI45_RS03910
• Protein ID: WP_000854677.1
• Coordinates: 784011..784379 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: V0SU84
• Locus tag: HYI45_RS03915
• Protein ID: WP_000065824.1
• Coordinates: 784400..784717 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYI45_RS03890 (779391)	779391..781034	+	1644	WP_001265639.1	fimbrial adhesin EcpD	-
HYI45_RS03895 (781003)	781003..781713	+	711	WP_001361803.1	fimbrial chaperone EcpE	-
HYI45_RS03900 (782026)	782026..782355	+	330	WP_001303809.1	YkgJ family cysteine cluster protein	-
HYI45_RS03905 (782604)	782604..783218	-	615	WP_001019920.1	YagU family protein	-
HYI45_RS03910 (784011)	784011..784379	-	369	WP_000854677.1	TA system toxin CbtA family protein	Toxin
HYI45_RS03915 (784400)	784400..784717	-	318	WP_000065824.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HYI45_RS03920 (784737)	784737..784958	-	222	WP_001220307.1	DUF987 domain-containing protein	-
HYI45_RS03925 (784967)	784967..785446	-	480	WP_000438147.1	RadC family protein	-
HYI45_RS03930 (785458)	785458..785916	-	459	WP_000211834.1	antirestriction protein	-
HYI45_RS03935 (786002)	786002..786238	-	237	Protein_772	DUF905 domain-containing protein	-
HYI45_RS03945 (787722)	787722..787873	-	152	Protein_774	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	756761..795703	38942

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13687.89 Da
• Isoelectric Point: 8.2671

>T167210 WP_000854677.1 NZ_CP058714:c784379-784011 [Escherichia coli]
MKTLPATTPQAAKPCLSPVSVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTTTDILQARKACGLMSRCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T167210 NZ_CP075061:4689232-4689552 [Escherichia coli]
ATGAAAACTCAACCTGCAACAACTCCGCAGGCGGCGAAGCCATGTCTGTCGCCCCTGGCTGTCTGGCAAATGTTACTGAC
AAGCCTGCTAGACCAGCACTATGGTCTCACGCTAAACGACACGCCGTTCAGTGATGAAACTGTTATTCAGAAACACATTG
AGGCAGGTATTACCTTGGCCGATGCCGTAAACTTTCTGGTTGAACGCTATGAACTAGTTCGTATTGACCAAAAAGGCTTC
TCTGTGCAAGATCAAGAACCTTGGTTAACCTCTATGGATGTTCACCGAGCACGATTTAAACTCAACGTGGAACGTTTATA
A

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11964.46 Da
• Isoelectric Point: 6.3163

>AT167210 WP_000065824.1 NZ_CP058714:c784717-784400 [Escherichia coli]
MSNITWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAEYRKLDETFPHFISQMESMLTTGEMNPRHAHCVTLYH
DGFTCEADTLGSCGYVYIVVYSTQR

Nucleotide

• Length: 318 bp

>AT167210 NZ_CP075061:4688885-4689211 [Escherichia coli]
ATGTCATTAATACCAGCCCATGAATGGGGACTACAAAGAGAAATTGTTCCACGGTTTGGAGCCAGATTAGTTCAGGAGGG
CAACCGGCTGCATTATCTGGCTGACAGGGCTAATTTGATGGGCAATTTCAGCGATACGGAATGCTTCAAATTGAATGACG
CATTTCCACACTTTATCAGTCAGATGGAATCGATGCTGACCACTGGTGAACTAATCCCCTGTCACCATCACTGTGTCACT
CTCTACCACAACGGTTTTACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTGTACATCGCGGTTTATCCCACTCA
ACGCTAA

Structures

Toxin

• AlphaFold DB: V0SXW1

Antitoxin

• AlphaFold DB: V0SU84