Detailed information of TA system TA167209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 628986..629823
• Replicon: chromosome
• Accession: NZ_CP058714
• Organism: Escherichia coli strain M172-1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: V0SED1
• Locus tag: HYI45_RS03170
• Protein ID: WP_000227787.1
• Coordinates: 629281..629823 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: HYI45_RS03165
• Protein ID: WP_001353405.1
• Coordinates: 628986..629297 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYI45_RS03140 (624006)	624006..624953	+	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-
HYI45_RS03145 (624975)	624975..626966	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HYI45_RS03150 (626956)	626956..627570	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HYI45_RS03155 (627570)	627570..627899	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HYI45_RS03160 (627911)	627911..628801	+	891	WP_000971336.1	heme o synthase	-
HYI45_RS03165 (628986)	628986..629297	+	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
HYI45_RS03170 (629281)	629281..629823	+	543	WP_000227787.1	GNAT family N-acetyltransferase	Toxin
HYI45_RS03175 (629879)	629879..630814	-	936	WP_012311701.1	tetratricopeptide repeat protein	-
HYI45_RS03180 (631221)	631221..632585	+	1365	WP_001000978.1	MFS transporter	-
HYI45_RS03185 (632713)	632713..633204	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
HYI45_RS03190 (633372)	633372..634283	+	912	WP_000705871.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19946.24 Da
• Isoelectric Point: 8.8951

>T167209 WP_000227787.1 NZ_CP058714:629281-629823 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T167209 NZ_CP075061:4136935-4137399 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT167209 WP_001353405.1 NZ_CP058714:628986-629297 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT167209 NZ_CP075061:4136600-4136935 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829GE43

Antitoxin

• AlphaFold DB: A0A829G7G3