Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 594120..594738 Replicon chromosome
Accession NZ_CP058714
Organism Escherichia coli strain M172-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HYI45_RS02990 Protein ID WP_001291435.1
Coordinates 594520..594738 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HYI45_RS02985 Protein ID WP_000344800.1
Coordinates 594120..594494 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYI45_RS02975 (589209) 589209..590402 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HYI45_RS02980 (590425) 590425..593574 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HYI45_RS02985 (594120) 594120..594494 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HYI45_RS02990 (594520) 594520..594738 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HYI45_RS02995 (594910) 594910..595461 + 552 WP_000102561.1 maltose O-acetyltransferase -
HYI45_RS03000 (595577) 595577..596047 + 471 WP_000136192.1 YlaC family protein -
HYI45_RS03005 (596211) 596211..596633 + 423 Protein_586 cyclic-guanylate-specific phosphodiesterase PdeB -
HYI45_RS03015 (597396) 597396..598538 + 1143 Protein_588 cyclic-guanylate-specific phosphodiesterase PdeB -
HYI45_RS03020 (598579) 598579..598932 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HYI45_RS03030 (599311) 599311..599622 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T167208 WP_001291435.1 NZ_CP058714:594520-594738 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T167208 NZ_CP075061:c3860061-3859654 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT167208 WP_000344800.1 NZ_CP058714:594120-594494 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT167208 NZ_CP075061:c3860308-3860042 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References