Detailed information of TA system TA167201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 39302..39824
• Replicon: plasmid pS163-2.2
• Accession: NZ_CP058711
• Organism: Escherichia coli strain S163-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0QMR1
• Locus tag: HZS88_RS23825
• Protein ID: WP_000220561.1
• Coordinates: 39543..39824 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: HZS88_RS23820
• Protein ID: WP_000121743.1
• Coordinates: 39302..39553 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS88_RS23795 (34541)	34541..35401	+	861	WP_063122221.1	hypothetical protein	-
HZS88_RS23800 (35422)	35422..35778	-	357	WP_001714234.1	DNA distortion polypeptide 3	-
HZS88_RS23805 (35915)	35915..36361	-	447	WP_001074384.1	hypothetical protein	-
HZS88_RS23810 (36365)	36365..37207	-	843	WP_000435064.1	replication initiation protein	-
HZS88_RS23815 (37228)	37228..38067	-	840	WP_001352874.1	replication initiation protein	-
HZS88_RS23820 (39302)	39302..39553	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
HZS88_RS23825 (39543)	39543..39824	+	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZS88_RS23830 (40174)	40174..40689	+	516	WP_001025387.1	J domain-containing protein	-
HZS88_RS23835 (40763)	40763..41311	+	549	WP_001178641.1	hypothetical protein	-
HZS88_RS23840 (41320)	41320..41589	+	270	WP_001006718.1	hypothetical protein	-
HZS88_RS23845 (41579)	41579..41821	+	243	WP_000008708.1	hypothetical protein	-
HZS88_RS23850 (41954)	41954..42658	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HZS88_RS23855 (42664)	42664..42804	+	141	WP_001044210.1	hypothetical protein	-
HZS88_RS23860 (43290)	43290..44027	+	738	WP_001446887.1	recombinase family protein	-
HZS88_RS23865 (44024)	44024..44248	+	225	WP_000743213.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / aac(3)-IId / floR / blaCTX-M-65	-	1..104573	104573

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11023.86 Da
• Isoelectric Point: 10.5938

>T167201 WP_000220561.1 NZ_CP058711:39543-39824 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Nucleotide

• Length: 282 bp

>T167201 NZ_CP075061:c1255525-1255307 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT167201 WP_000121743.1 NZ_CP058711:39302-39553 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT167201 NZ_CP075061:c1255925-1255551 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A3S5I302

Antitoxin

• AlphaFold DB: A0A3S5I301