Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3705201..3705855 Replicon chromosome
Accession NZ_CP058709
Organism Escherichia coli strain S163-2

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HZS88_RS18510 Protein ID WP_000244777.1
Coordinates 3705448..3705855 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZS88_RS18505 Protein ID WP_000354046.1
Coordinates 3705201..3705467 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS88_RS18480 (3700370) 3700370..3701113 + 744 WP_000951964.1 SDR family oxidoreductase -
HZS88_RS18485 (3701170) 3701170..3702603 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
HZS88_RS18490 (3702648) 3702648..3702959 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HZS88_RS18495 (3703123) 3703123..3703782 + 660 WP_000250274.1 hemolysin III family protein -
HZS88_RS18500 (3703978) 3703978..3704958 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HZS88_RS18505 (3705201) 3705201..3705467 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZS88_RS18510 (3705448) 3705448..3705855 + 408 WP_000244777.1 protein YgfX Toxin
HZS88_RS18515 (3705895) 3705895..3706416 - 522 WP_001055874.1 flavodoxin FldB -
HZS88_RS18520 (3706528) 3706528..3707424 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HZS88_RS18525 (3707449) 3707449..3708159 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZS88_RS18530 (3708165) 3708165..3709898 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T167199 WP_000244777.1 NZ_CP058709:3705448-3705855 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T167199 NZ_CP075061:c990820-990542 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT167199 WP_000354046.1 NZ_CP058709:3705201-3705467 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT167199 NZ_CP075061:c991083-990823 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References