Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2423774..2424271 Replicon chromosome
Accession NZ_CP058709
Organism Escherichia coli strain S163-2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HZS88_RS12265 Protein ID WP_001173343.1
Coordinates 2423774..2423947 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag HZS88_RS12270 Protein ID WP_001425728.1
Coordinates 2423975..2424271 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS88_RS12255 (2420444) 2420444..2421901 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HZS88_RS12260 (2422138) 2422138..2423655 + 1518 WP_001295074.1 lysine--tRNA ligase -
HZS88_RS12265 (2423774) 2423774..2423947 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HZS88_RS12270 (2423975) 2423975..2424271 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HZS88_RS12275 (2424498) 2424498..2424770 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
HZS88_RS12280 (2424782) 2424782..2425012 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
HZS88_RS12285 (2425193) 2425193..2426824 + 1632 WP_001216486.1 sensor histidine kinase -
HZS88_RS12290 (2426821) 2426821..2427540 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T167196 WP_001173343.1 NZ_CP058709:c2423947-2423774 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T167196 NZ_CP075061:136155-136466 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT167196 WP_001425728.1 NZ_CP058709:c2424271-2423975 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT167196 NZ_CP075061:136467-136757 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References