Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2225752..2226328 Replicon chromosome
Accession NZ_CP058709
Organism Escherichia coli strain S163-2

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HZS88_RS11280 Protein ID WP_001297643.1
Coordinates 2226041..2226328 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag HZS88_RS11275 Protein ID WP_000063160.1
Coordinates 2225752..2226054 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS88_RS11260 (2222348) 2222348..2224498 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
HZS88_RS11265 (2224548) 2224548..2224751 + 204 WP_000467859.1 YbdD/YjiX family protein -
HZS88_RS11270 (2224762) 2224762..2225718 + 957 WP_001297640.1 GTPase -
HZS88_RS11275 (2225752) 2225752..2226054 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
HZS88_RS11280 (2226041) 2226041..2226328 - 288 WP_001297643.1 BrnT family toxin Toxin
HZS88_RS25300 (2226527) 2226527..2227223 - 697 Protein_2214 IS1 family transposase -
HZS88_RS11295 (2227538) 2227538..2228770 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
HZS88_RS11300 (2228811) 2228811..2230091 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2226527..2226736 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T167195 WP_001297643.1 NZ_CP058709:c2226328-2226041 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T167195 NZ_CP075060:c43379-43227 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT167195 WP_000063160.1 NZ_CP058709:c2226054-2225752 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167195 NZ_CP075060:43431-43491 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References