Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 18701..19345 Replicon plasmid unnamed3
Accession NZ_CP058685
Organism Escherichia coli strain MBT-5

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag HZT35_RS30085 Protein ID WP_000833471.1
Coordinates 18701..18883 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag HZT35_RS30090 Protein ID WP_000466317.1
Coordinates 18908..19345 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT35_RS30055 13738..14751 - 1014 WP_024176825.1 replication initiation protein -
HZT35_RS30060 16388..16612 + 225 WP_000749828.1 hypothetical protein -
HZT35_RS30065 16663..16929 + 267 WP_000868277.1 hypothetical protein -
HZT35_RS30070 16919..17161 + 243 WP_001450803.1 hypothetical protein -
HZT35_RS30075 17602..17931 + 330 WP_021558913.1 hypothetical protein -
HZT35_RS30080 17989..18267 + 279 WP_136748664.1 cobalamin biosynthesis protein CbiX -
HZT35_RS30085 18701..18883 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
HZT35_RS30090 18908..19345 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HZT35_RS30095 19374..20078 - 705 WP_032274884.1 hypothetical protein -
HZT35_RS30100 20075..20836 - 762 WP_001097938.1 conjugal transfer protein TraL -
HZT35_RS30105 20846..21253 - 408 WP_001190621.1 hypothetical protein -
HZT35_RS30110 21599..21934 + 336 WP_000963832.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..34196 34196


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T167171 WP_000833471.1 NZ_CP058685:18701-18883 [Escherichia coli]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T167171 NZ_CP075037:200885-201370 [Salmonella enterica subsp. enterica serovar Give]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACTTTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT167171 WP_000466317.1 NZ_CP058685:18908-19345 [Escherichia coli]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT167171 NZ_CP075037:200610-200897 [Salmonella enterica subsp. enterica serovar Give]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References