Detailed information of TA system TA167169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 81795..82396
• Replicon: plasmid unnamed2
• Accession: NZ_CP058684
• Organism: Escherichia coli strain MBT-5

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HZT35_RS29890
• Protein ID: WP_044705796.1
• Coordinates: 81795..82175 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: HZT35_RS29895
• Protein ID: WP_001190712.1
• Coordinates: 82175..82396 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT35_RS29865	77236..78720	-	1485	WP_000124159.1	hypothetical protein	-
HZT35_RS29870	78720..79913	-	1194	WP_048251898.1	hypothetical protein	-
HZT35_RS29875	79999..80451	-	453	WP_001326849.1	late promoter-activating protein	-
HZT35_RS29880	80540..81583	-	1044	WP_000648833.1	DUF968 domain-containing protein	-
HZT35_RS29885	81611..81790	-	180	WP_000113018.1	hypothetical protein	-
HZT35_RS29890	81795..82175	-	381	WP_044705796.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HZT35_RS29895	82175..82396	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HZT35_RS29900	82505..82927	-	423	WP_000098854.1	hypothetical protein	-
HZT35_RS29905	83062..83451	-	390	WP_000506729.1	DNA repair protein	-
HZT35_RS29910	83573..83851	-	279	WP_136748066.1	hypothetical protein	-
HZT35_RS29915	84124..84774	-	651	WP_182111099.1	hypothetical protein	-
HZT35_RS29920	84756..85130	-	375	WP_109475749.1	hypothetical protein	-
HZT35_RS29925	85137..85430	-	294	WP_000267997.1	hypothetical protein	-
HZT35_RS29930	85454..85735	-	282	WP_000002123.1	ASCH domain-containing protein	-
HZT35_RS29935	85735..85995	-	261	WP_000969524.1	hypothetical protein	-
HZT35_RS29940	85992..86849	-	858	WP_024231017.1	DUF551 domain-containing protein	-
HZT35_RS29945	86846..87178	-	333	WP_157847308.1	hypothetical protein	-
HZT35_RS29950	87183..87361	-	179	Protein_101	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89589	89589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T167169 WP_044705796.1 NZ_CP058684:c82175-81795 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEISDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDTPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T167169 NZ_CP074714:270373-270723 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT167169 WP_001190712.1 NZ_CP058684:c82396-82175 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT167169 NZ_CP074714:270081-270368 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6