Detailed information of TA system TA167168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 82372..83015
• Replicon: plasmid unnamed1
• Accession: NZ_CP058683
• Organism: Escherichia coli strain MBT-5

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HZT35_RS29365
• Protein ID: WP_024017568.1
• Coordinates: 82599..83015 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: HZT35_RS29360
• Protein ID: WP_001261287.1
• Coordinates: 82372..82602 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT35_RS29330	78237..79676	+	1440	WP_001213545.1	enterohemolysin T1SS ABC transporter subunit EhxD	-
HZT35_RS29335	79743..79886	+	144	Protein_75	transcriptional regulator	-
HZT35_RS29340	79890..80081	+	192	Protein_76	lipid II-degrading bacteriocin	-
HZT35_RS29345	80089..80442	-	354	WP_000864810.1	colicin M immunity protein	-
HZT35_RS29350	80615..81397	-	783	WP_001164205.1	site-specific integrase	-
HZT35_RS29355	81399..81812	-	414	WP_000465041.1	hypothetical protein	-
HZT35_RS29360	82372..82602	+	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HZT35_RS29365	82599..83015	+	417	WP_024017568.1	type II toxin-antitoxin system VapC family toxin	Toxin
HZT35_RS29370	83177..83722	-	546	WP_001165114.1	hypothetical protein	-
HZT35_RS29375	83790..84947	+	1158	WP_106914028.1	IS3-like element IS600 family transposase	-
HZT35_RS29380	84988..85137	-	150	WP_000955366.1	hypothetical protein	-
HZT35_RS29385	85752..86612	-	861	WP_000704534.1	alpha/beta hydrolase	-
HZT35_RS29390	86740..87126	+	387	WP_001066949.1	tyrosine-type recombinase/integrase	-
HZT35_RS29395	87180..87854	+	675	WP_001341423.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	toxB / espP / hlyC / hlyA / hlyB / hlyD	1..92578	92578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15196.72 Da
• Isoelectric Point: 8.7503

>T167168 WP_024017568.1 NZ_CP058683:82599-83015 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDKFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T167168 NZ_CP074712:c9623-9330 [Acinetobacter baumannii]
ATGACTTATAAGCTTTTACGTCACAAGGACTTTACAGCAGAATGGGAAAAACTCCCTGTTGCTATACGGGATCAATTTAA
GAAGAAACTAGCCAAAGTCATAGAGCAGCCACATATTCCAAAGAATATGCTGAGAGGCGATCTTGCAGGTTGCTATAAAA
TTAAATTATTAAAAGCTGGTGTCAGACTTGTCTATCAAGTTAAAGATGATCAGGTAGTTATCTTGCTCATCACAGTCGGG
AAACGAGCTGATAGCATAGTTTATGACGAAGCTAAAAAGCGCATTAAAGACTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT167168 WP_001261287.1 NZ_CP058683:82372-82602 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT167168 NZ_CP074712:c9858-9613 [Acinetobacter baumannii]
ATGGCTACTGTTAACTTCAGGGTCGATGAAGCCCTTAAAGAAAAATCCTATTCCATCCTTAAAGAACAAGGTATTGCACC
TACAGATTTCTTTACTAGTATTCTTGAGTATGTTGCTACGACTGGGAAACTACCTGTCAAAAAAGCTTTGCTTTCAGAAG
AAGATGAAGAATTGTTAGCTCTTGTCCGTAAGCGCATTAATGACCCTAAAGAGATGTTTGAGGAAGTCACATTAGATGAC
TTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QFC4