Detailed information of TA system TA167164

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4957491..4958323
• Replicon: chromosome
• Accession: NZ_CP058682
• Organism: Escherichia coli strain MBT-5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: HZT35_RS25420
• Protein ID: WP_000854753.1
• Coordinates: 4957491..4957865 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: HZT35_RS25425
• Protein ID: WP_001315620.1
• Coordinates: 4957955..4958323 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT35_RS25390	4952706..4953812	+	1107	WP_182111086.1	N-acetylneuraminate epimerase	-
HZT35_RS25395	4953877..4954857	+	981	WP_000991415.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
HZT35_RS25400	4954865..4955515	-	651	WP_001037966.1	HNH endonuclease	-
HZT35_RS25405	4956560..4956712	-	153	WP_001280445.1	hypothetical protein	-
HZT35_RS25410	4956797..4956994	-	198	WP_000839281.1	DUF957 domain-containing protein	-
HZT35_RS25415	4957006..4957494	-	489	WP_000777547.1	hypothetical protein	-
HZT35_RS25420	4957491..4957865	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
HZT35_RS25425	4957955..4958323	-	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HZT35_RS25430	4958486..4958707	-	222	WP_000692345.1	DUF987 domain-containing protein	-
HZT35_RS25435	4958770..4959246	-	477	WP_001186774.1	RadC family protein	-
HZT35_RS25440	4959262..4959747	-	486	WP_000849588.1	antirestriction protein	-
HZT35_RS25445	4959802..4960620	-	819	WP_001234622.1	DUF945 domain-containing protein	-
HZT35_RS25450	4960720..4960953	-	234	WP_001119729.1	DUF905 family protein	-
HZT35_RS25455	4961032..4961487	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T167164 WP_000854753.1 NZ_CP058682:c4957865-4957491 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T167164 NZ_CP074711:13831-14187 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGTACAGCAAGATGAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ACATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCATTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAACT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT167164 WP_001315620.1 NZ_CP058682:c4958323-4957955 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT167164 NZ_CP074711:14180-14482 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCGTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTTAATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5