167162

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4355239..4356076 Replicon chromosome
Accession NZ_CP058682
Organism Escherichia coli strain MBT-5

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag HZT35_RS22570 Protein ID WP_000227784.1
Coordinates 4355534..4356076 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HZT35_RS22565 Protein ID WP_001297137.1
Coordinates 4355239..4355550 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT35_RS22540 4350259..4351206 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
HZT35_RS22545 4351228..4353219 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HZT35_RS22550 4353209..4353823 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HZT35_RS22555 4353823..4354152 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HZT35_RS22560 4354164..4355054 + 891 WP_000971336.1 heme o synthase -
HZT35_RS22565 4355239..4355550 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HZT35_RS22570 4355534..4356076 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
HZT35_RS22575 4356132..4357067 - 936 WP_001375746.1 sel1 repeat family protein -
HZT35_RS22580 4357475..4358839 + 1365 WP_001000977.1 MFS transporter -
HZT35_RS22585 4358967..4359458 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HZT35_RS22590 4359626..4360537 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T167162 WP_000227784.1 NZ_CP058682:4355534-4356076 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T167162 NZ_CP074710:1888291-1888473 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT167162 WP_001297137.1 NZ_CP058682:4355239-4355550 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT167162 NZ_CP074710:1888566-1888970 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References