Detailed information of TA system TA167160

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3420515..3421310
• Replicon: chromosome
• Accession: NZ_CP058682
• Organism: Escherichia coli strain MBT-5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: HZT35_RS17665
• Protein ID: WP_000854914.1
• Coordinates: 3420515..3420889 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9LM11
• Locus tag: HZT35_RS17670
• Protein ID: WP_001280953.1
• Coordinates: 3420936..3421310 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT35_RS17625	3415519..3416010	-	492	WP_001297187.1	DUF1097 domain-containing protein	-
HZT35_RS17630	3416112..3416666	-	555	WP_001001902.1	molecular chaperone YcdY	-
HZT35_RS17635	3416690..3417427	-	738	WP_000283664.1	zinc-binding phosphatase	-
HZT35_RS17640	3417482..3418420	-	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
HZT35_RS17650	3418891..3419733	-	843	WP_001280481.1	DUF4942 domain-containing protein	-
HZT35_RS17655	3419818..3420015	-	198	WP_000839282.1	DUF957 domain-containing protein	-
HZT35_RS17660	3420027..3420518	-	492	WP_000976857.1	hypothetical protein	-
HZT35_RS17665	3420515..3420889	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
HZT35_RS17670	3420936..3421310	-	375	WP_001280953.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HZT35_RS17675	3421473..3421694	-	222	WP_000692323.1	DUF987 domain-containing protein	-
HZT35_RS17680	3421757..3422233	-	477	WP_001186738.1	RadC family protein	-
HZT35_RS17685	3422249..3422734	-	486	WP_000214398.1	antirestriction protein	-
HZT35_RS17690	3422825..3423643	-	819	WP_001234682.1	DUF945 domain-containing protein	-
HZT35_RS17695	3423983..3425053	-	1071	WP_000102669.1	patatin-like phospholipase family protein	-
HZT35_RS17700	3425050..3425955	-	906	WP_000203541.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgC / csgA / csgB / csgD / csgE / csgF / csgG	3411014..3422734	11720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T167160 WP_000854914.1 NZ_CP058682:c3420889-3420515 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T167160 NZ_CP074707:12028-12315 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13734.56 Da
• Isoelectric Point: 6.6249

>AT167160 WP_001280953.1 NZ_CP058682:c3421310-3420936 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS

Nucleotide

• Length: 375 bp

>AT167160 NZ_CP074707:12302-12616 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTAAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A7U9LM11