Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2612956..2613594 Replicon chromosome
Accession NZ_CP058682
Organism Escherichia coli strain MBT-5

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HZT35_RS13290 Protein ID WP_000813794.1
Coordinates 2612956..2613132 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HZT35_RS13295 Protein ID WP_001270286.1
Coordinates 2613178..2613594 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT35_RS13270 2608575..2609789 - 1215 WP_001307190.1 BenE family transporter YdcO -
HZT35_RS13275 2609842..2610378 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HZT35_RS13280 2610451..2612412 + 1962 WP_044862087.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HZT35_RS13285 2612504..2612734 - 231 WP_000494244.1 YncJ family protein -
HZT35_RS13290 2612956..2613132 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HZT35_RS13295 2613178..2613594 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HZT35_RS13300 2613673..2615078 + 1406 Protein_2597 PLP-dependent aminotransferase family protein -
HZT35_RS13305 2615323..2616468 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
HZT35_RS13310 2616486..2617499 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
HZT35_RS13315 2617500..2618441 + 942 WP_001251332.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2607387..2608535 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T167157 WP_000813794.1 NZ_CP058682:2612956-2613132 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T167157 NZ_CP074704:58979-59359 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATGATCCCGTGCTGGTGGAGCTTGCAGTGGGGGCTGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGAAAAAACTACGTGAACTATATGGTTCCAATATTTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT167157 WP_001270286.1 NZ_CP058682:2613178-2613594 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT167157 NZ_CP074704:58758-58979 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAACCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACACTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References