Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2042395..2043230 Replicon chromosome
Accession NZ_CP058682
Organism Escherichia coli strain MBT-5

Toxin (Protein)


Gene name yeeV Uniprot ID A0A136X5A2
Locus tag HZT35_RS10140 Protein ID WP_000854757.1
Coordinates 2042395..2042772 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag HZT35_RS10145 Protein ID WP_001295723.1
Coordinates 2042862..2043230 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT35_RS10105 2037915..2038388 + 474 WP_001105384.1 DNA gyrase inhibitor SbmC -
HZT35_RS10110 2038586..2039644 + 1059 WP_001200883.1 FUSC family protein -
HZT35_RS10115 2039816..2040145 + 330 WP_000450409.1 DUF496 family protein -
HZT35_RS10120 2040246..2040428 - 183 WP_024167529.1 ethanolamine utilization protein -
HZT35_RS10125 2040882..2041910 - 1029 Protein_1983 IS110 family transposase -
HZT35_RS10130 2042069..2042182 - 114 WP_001161660.1 DUF957 domain-containing protein -
HZT35_RS10135 2042195..2042398 - 204 WP_000761705.1 hypothetical protein -
HZT35_RS10140 2042395..2042772 - 378 WP_000854757.1 TA system toxin CbtA family protein Toxin
HZT35_RS10145 2042862..2043230 - 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HZT35_RS10150 2043393..2043614 - 222 WP_000692323.1 DUF987 domain-containing protein -
HZT35_RS10155 2043683..2044159 - 477 WP_001186725.1 RadC family protein -
HZT35_RS10160 2044175..2044660 - 486 WP_000849582.1 antirestriction protein -
HZT35_RS10165 2044715..2045533 - 819 WP_001234620.1 DUF945 domain-containing protein -
HZT35_RS10170 2045633..2045866 - 234 WP_001119729.1 DUF905 family protein -
HZT35_RS10175 2045945..2046286 - 342 WP_072097794.1 hypothetical protein -
HZT35_RS10180 2046375..2046779 + 405 WP_000839179.1 IS66 family insertion sequence hypothetical protein -
HZT35_RS10185 2046776..2047123 + 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14153.18 Da        Isoelectric Point: 7.3249

>T167141 WP_000854757.1 NZ_CP058682:c2042772-2042395 [Escherichia coli]
MKTLPDTHVREVSCCPSLVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T167141 NZ_CP074701:c2946943-2946836 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTCGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT167141 WP_001295723.1 NZ_CP058682:c2043230-2042862 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT167141 NZ_CP074701:2947000-2947055 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A136X5A2

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References