Detailed information of TA system TA167139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1385182..1385807
• Replicon: chromosome
• Accession: NZ_CP058682
• Organism: Escherichia coli strain MBT-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HZT35_RS06765
• Protein ID: WP_000911330.1
• Coordinates: 1385409..1385807 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HZT35_RS06760
• Protein ID: WP_000450524.1
• Coordinates: 1385182..1385409 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT35_RS06735	1380985..1381455	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HZT35_RS06740	1381455..1382027	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HZT35_RS06745	1382173..1383051	+	879	WP_044861983.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HZT35_RS06750	1383068..1384102	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
HZT35_RS06755	1384315..1385028	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HZT35_RS06760	1385182..1385409	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HZT35_RS06765	1385409..1385807	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HZT35_RS06770	1385954..1386817	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
HZT35_RS06775	1386832..1388846	+	2015	Protein_1324	tRNA cytosine(34) acetyltransferase TmcA	-
HZT35_RS06780	1388920..1389618	+	699	WP_000679812.1	esterase	-
HZT35_RS06785	1389728..1389928	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T167139 WP_000911330.1 NZ_CP058682:1385409-1385807 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T167139 NZ_CP074701:2312230-2312607 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT167139 WP_000450524.1 NZ_CP058682:1385182-1385409 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT167139 NZ_CP074701:2311809-2312183 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33