Detailed information of TA system TA167098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 2181774..2182882
• Replicon: chromosome
• Accession: NZ_CP058666
• Organism: Streptococcus agalactiae strain S9968

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A3S8RBH6
• Locus tag: S9968_RS11165
• Protein ID: WP_071661597.1
• Coordinates: 2181774..2182643 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: S9968_RS11170
• Protein ID: WP_000205227.1
• Coordinates: 2182658..2182882 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S9968_RS11135	2177545..2178225	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
S9968_RS11140	2178284..2178895	-	612	Protein_2123	aminoglycoside 6-adenylyltransferase	-
S9968_RS11145	2179065..2180504	-	1440	WP_001028144.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-
S9968_RS11150	2180505..2180897	-	393	WP_000393259.1	GNAT family N-acetyltransferase	-
S9968_RS11155	2180910..2181026	-	117	Protein_2126	aminoglycoside 6-adenylyltransferase	-
S9968_RS11160	2181059..2181793	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
S9968_RS11165	2181774..2182643	-	870	WP_071661597.1	nucleotidyltransferase domain-containing protein	Toxin
S9968_RS11170	2182658..2182882	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
S9968_RS11175	2183025..2183159	-	135	Protein_2130	site-specific recombinase	-
S9968_RS11180	2183300..2184601	+	1302	Protein_2131	ISL3 family transposase	-
S9968_RS11185	2184910..2185713	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
S9968_RS11190	2185767..2187251	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	lnu(B) / lsa(E)	-	2181774..2200876	19102
	-	inside	IScluster/Tn	aac(6')-aph(2'') / lnu(B) / lsa(E)	-	2177545..2189411	11866

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32902.55 Da
• Isoelectric Point: 4.9770

>T167098 WP_071661597.1 NZ_CP058666:c2182643-2181774 [Streptococcus agalactiae]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T167098 NZ_CP074680:998818-999153 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT167098 WP_000205227.1 NZ_CP058666:c2182882-2182658 [Streptococcus agalactiae]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT167098 NZ_CP074680:998570-998818 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A3S8RBH6