Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4602065..4602719 Replicon chromosome
Accession NZ_CP058661
Organism Escherichia coli strain 005008

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HZR83_RS22005 Protein ID WP_000244781.1
Coordinates 4602065..4602472 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZR83_RS22010 Protein ID WP_000354046.1
Coordinates 4602453..4602719 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZR83_RS21985 4598022..4599755 - 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -
HZR83_RS21990 4599761..4600471 - 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZR83_RS21995 4600496..4601392 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HZR83_RS22000 4601504..4602025 + 522 WP_001521233.1 flavodoxin FldB -
HZR83_RS22005 4602065..4602472 - 408 WP_000244781.1 protein YgfX Toxin
HZR83_RS22010 4602453..4602719 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZR83_RS22015 4602962..4603942 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
HZR83_RS22020 4604019..4604678 - 660 WP_000250269.1 hemolysin III family protein -
HZR83_RS22025 4604842..4605153 - 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
HZR83_RS22030 4605198..4606631 + 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T167079 WP_000244781.1 NZ_CP058661:c4602472-4602065 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T167079 NZ_CP074677:659427-659768 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCTACAATTTCGCGTGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGCCTGGCAAATGTTACTGAC
ACGCCTACTGGAACAGCACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACAGTTATTAAGGAACATATTG
ATGCCGAGATTACCCTGGCTGATGCAGTCAACTTCTTGGTGGATAAGTACGGACTGGTTCGTATCGATCGCAGGGGGTTT
AACTGGCAGGAACAATCGCCTTATCTTCGGGCTGTAGATATTCTGCGAGCACGACAGGCAACTGGCTTGCTGCGACGGAG
CCGTAATAATGCTGTATCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT167079 WP_000354046.1 NZ_CP058661:c4602719-4602453 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT167079 NZ_CP074677:659065-659406 [Klebsiella pneumoniae]
ATGAAATCTACTGAGTCAGAGAACGATTTATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGCATTCAGTAACGCAGAATCTT
TGAAGTTGGATGAAGTATTCCCACGTTTTATCAGCCAGATGGAATCGATGTTGACCATTGGTGAAATGAATCCTCACCAT
GCCCACTGCGTCACCCTATACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References