Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1456208..1456784 Replicon chromosome
Accession NZ_CP058661
Organism Escherichia coli strain 005008

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HZR83_RS06985 Protein ID WP_001297643.1
Coordinates 1456208..1456495 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag HZR83_RS06990 Protein ID WP_000063160.1
Coordinates 1456482..1456784 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZR83_RS06970 1452445..1453725 - 1281 WP_001037390.1 DUF445 domain-containing protein -
HZR83_RS06975 1453766..1454998 - 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
HZR83_RS06980 1455313..1456009 + 697 Protein_1352 IS1 family transposase -
HZR83_RS06985 1456208..1456495 + 288 WP_001297643.1 BrnT family toxin Toxin
HZR83_RS06990 1456482..1456784 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
HZR83_RS06995 1456818..1457774 - 957 WP_001297640.1 GTPase -
HZR83_RS07000 1457785..1457988 - 204 WP_000467859.1 YbdD/YjiX family protein -
HZR83_RS07005 1458038..1460188 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1455800..1456009 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T167061 WP_001297643.1 NZ_CP058661:1456208-1456495 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T167061 NZ_CP074663:2117054-2117157 [Salmonella enterica subsp. enterica serovar Typhimurium]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT167061 WP_000063160.1 NZ_CP058661:1456482-1456784 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167061 NZ_CP074663:c2117159-2117014 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References