Detailed information of TA system TA167056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 63579..64180
• Replicon: plasmid p2_035125
• Accession: NZ_CP058659
• Organism: Escherichia coli strain WCHEC035125

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HZR25_RS23895
• Protein ID: WP_112031922.1
• Coordinates: 63800..64180 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: HZR25_RS23890
• Protein ID: WP_001190712.1
• Coordinates: 63579..63800 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZR25_RS23850	59177..59866	+	690	WP_112031926.1	hypothetical protein	-
HZR25_RS23855	59853..60539	+	687	WP_080028522.1	ead/Ea22-like family protein	-
HZR25_RS23860	60536..60982	+	447	WP_112031925.1	ead/Ea22-like family protein	-
HZR25_RS23865	61155..61346	+	192	WP_021517457.1	hypothetical protein	-
HZR25_RS23870	61348..61611	+	264	WP_000224218.1	hypothetical protein	-
HZR25_RS23875	61622..62452	+	831	WP_112031924.1	DUF551 domain-containing protein	-
HZR25_RS23880	62767..63018	+	252	WP_112031923.1	DNA polymerase III subunit theta	-
HZR25_RS23885	63117..63506	+	390	WP_000506731.1	hypothetical protein	-
HZR25_RS23890	63579..63800	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HZR25_RS23895	63800..64180	+	381	WP_112031922.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HZR25_RS23900	64216..65310	-	1095	WP_112031921.1	hypothetical protein	-
HZR25_RS23905	65300..66064	-	765	WP_112031920.1	hypothetical protein	-
HZR25_RS23910	66071..66772	-	702	WP_089477112.1	DUF2829 domain-containing protein	-
HZR25_RS23915	66787..67281	-	495	WP_112031919.1	dUTP diphosphatase	-
HZR25_RS23920	67278..67754	-	477	WP_000861173.1	hypothetical protein	-
HZR25_RS23925	67751..68059	-	309	WP_000349257.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89623	89623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13637.50 Da
• Isoelectric Point: 5.6342

>T167056 WP_112031922.1 NZ_CP058659:63800-64180 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRDLYGSNI

Nucleotide

• Length: 381 bp

>T167056 NZ_CP074663:317267-317635 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT167056 WP_001190712.1 NZ_CP058659:63579-63800 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT167056 NZ_CP074663:317049-317270 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6