Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 98720..99245 Replicon plasmid p1_035125
Accession NZ_CP058658
Organism Escherichia coli strain WCHEC035125

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HZR25_RS23370 Protein ID WP_001159871.1
Coordinates 98720..99025 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag HZR25_RS23375 Protein ID WP_000813630.1
Coordinates 99027..99245 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZR25_RS23355 94682..95848 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HZR25_RS23360 96436..97191 - 756 WP_000852146.1 replication initiation protein RepE -
HZR25_RS23365 97913..98719 - 807 WP_000016970.1 site-specific integrase -
HZR25_RS23370 98720..99025 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HZR25_RS23375 99027..99245 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HZR25_RS23380 99805..100035 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HZR25_RS23385 100032..100448 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
HZR25_RS23390 100523..102088 + 1566 WP_001128474.1 AAA family ATPase -
HZR25_RS23395 102073..103095 + 1023 WP_000361402.1 helicase UvrD -
HZR25_RS23400 103349..104046 - 698 Protein_118 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / qnrS1 / tet(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..122305 122305
- flank IS/Tn - - 103349..103852 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T167053 WP_001159871.1 NZ_CP058658:c99025-98720 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T167053 NZ_CP074661:4479350-4479661 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACACTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGCACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATGATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT167053 WP_000813630.1 NZ_CP058658:c99245-99027 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT167053 NZ_CP074661:4479658-4480104 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References