Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4206878..4207375 Replicon chromosome
Accession NZ_CP058656
Organism Escherichia coli strain WCHEC035125

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HZR25_RS20350 Protein ID WP_001173343.1
Coordinates 4206878..4207051 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag HZR25_RS20355 Protein ID WP_001425728.1
Coordinates 4207079..4207375 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZR25_RS20340 4203548..4205005 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
HZR25_RS20345 4205242..4206759 + 1518 WP_001295074.1 lysine--tRNA ligase -
HZR25_RS20350 4206878..4207051 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HZR25_RS20355 4207079..4207375 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HZR25_RS20360 4207602..4207874 - 273 WP_000405647.1 N-acetyltransferase -
HZR25_RS20365 4207886..4208116 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
HZR25_RS20370 4208297..4209928 + 1632 WP_001216487.1 sensor histidine kinase -
HZR25_RS20375 4209925..4210644 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T167044 WP_001173343.1 NZ_CP058656:c4207051-4206878 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T167044 NZ_CP074661:c2431500-2431345 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT167044 WP_001425728.1 NZ_CP058656:c4207375-4207079 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT167044 NZ_CP074661:2431512-2431570 [Salmonella enterica subsp. enterica serovar Enteritidis]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References