Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 316941..317741 Replicon chromosome
Accession NZ_CP058656
Organism Escherichia coli strain WCHEC035125

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag HZR25_RS01445 Protein ID WP_000342449.1
Coordinates 317214..317741 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag HZR25_RS01440 Protein ID WP_001277108.1
Coordinates 316941..317207 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZR25_RS01420 312598..313266 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
HZR25_RS01425 313259..314317 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
HZR25_RS01430 314562..315416 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
HZR25_RS01435 315688..316791 + 1104 WP_112031961.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
HZR25_RS01440 316941..317207 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
HZR25_RS01445 317214..317741 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
HZR25_RS01450 317738..318121 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
HZR25_RS01455 318545..319654 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
HZR25_RS01460 319702..320628 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HZR25_RS01465 320625..321902 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
HZR25_RS01470 321899..322666 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T167025 WP_000342449.1 NZ_CP058656:317214-317741 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T167025 NZ_CP074606:197333-197818 [Salmonella enterica subsp. enterica serovar Newport str.]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT167025 WP_001277108.1 NZ_CP058656:316941-317207 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT167025 NZ_CP074606:197058-197345 [Salmonella enterica subsp. enterica serovar Newport str.]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References