Detailed information of TA system TA167024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 48202..48803
• Replicon: plasmid p2_035123
• Accession: NZ_CP058654
• Organism: Escherichia coli strain WCHEC035123

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HZR24_RS23980
• Protein ID: WP_112031922.1
• Coordinates: 48423..48803 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: HZR24_RS23975
• Protein ID: WP_001190712.1
• Coordinates: 48202..48423 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZR24_RS23935	43800..44489	+	690	WP_112031926.1	hypothetical protein	-
HZR24_RS23940	44476..45162	+	687	WP_080028522.1	ead/Ea22-like family protein	-
HZR24_RS23945	45159..45605	+	447	WP_112031925.1	ead/Ea22-like family protein	-
HZR24_RS23950	45778..45969	+	192	WP_021517457.1	hypothetical protein	-
HZR24_RS23955	45971..46234	+	264	WP_000224218.1	hypothetical protein	-
HZR24_RS23960	46245..47075	+	831	WP_112031924.1	DUF551 domain-containing protein	-
HZR24_RS23965	47390..47641	+	252	WP_112031923.1	DNA polymerase III subunit theta	-
HZR24_RS23970	47740..48129	+	390	WP_000506731.1	hypothetical protein	-
HZR24_RS23975	48202..48423	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HZR24_RS23980	48423..48803	+	381	WP_112031922.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HZR24_RS23985	48839..49933	-	1095	WP_112031921.1	hypothetical protein	-
HZR24_RS23990	49923..50687	-	765	WP_112031920.1	hypothetical protein	-
HZR24_RS23995	50694..51395	-	702	WP_089477112.1	DUF2829 domain-containing protein	-
HZR24_RS24000	51410..51904	-	495	WP_112031919.1	dUTP diphosphatase	-
HZR24_RS24005	51901..52377	-	477	WP_000861173.1	hypothetical protein	-
HZR24_RS24010	52374..52682	-	309	WP_000349257.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89623	89623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13637.50 Da
• Isoelectric Point: 5.6342

>T167024 WP_112031922.1 NZ_CP058654:48423-48803 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRDLYGSNI

Nucleotide

• Length: 381 bp

>T167024 NZ_CP074592:c4318963-4318652 [Salmonella bongori serovar 40:z35:-]
ATGCAATTCATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGCAGTGGCGTACGAATTATCTATTTTCATCGTAGTCAACGGTATGAGGTTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT167024 WP_001190712.1 NZ_CP058654:48202-48423 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT167024 NZ_CP074592:c4318651-4318361 [Salmonella bongori serovar 40:z35:-]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGATCCGAGAAACGTTATACAAGCATTGCGTCATTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6