Detailed information of TA system TA167018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 138966..139609
• Replicon: plasmid p1_035123
• Accession: NZ_CP058653
• Organism: Escherichia coli strain WCHEC035123

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: V0UN72
• Locus tag: HZR24_RS23555
• Protein ID: WP_001034044.1
• Coordinates: 139193..139609 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B1P7N7
• Locus tag: HZR24_RS23550
• Protein ID: WP_001261286.1
• Coordinates: 138966..139196 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZR24_RS23530	135597..136352	-	756	WP_000852146.1	replication initiation protein RepE	-
HZR24_RS23535	137074..137880	-	807	WP_000016970.1	site-specific integrase	-
HZR24_RS23540	137881..138186	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	-
HZR24_RS23545	138188..138406	-	219	WP_000813630.1	type II toxin-antitoxin system antitoxin CcdA	-
HZR24_RS23550	138966..139196	+	231	WP_001261286.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HZR24_RS23555	139193..139609	+	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	Toxin
HZR24_RS23560	139684..141249	+	1566	WP_001128474.1	AAA family ATPase	-
HZR24_RS23565	141234..142256	+	1023	WP_000361402.1	helicase UvrD	-
HZR24_RS23570	142510..143207	-	698	Protein_155	IS1-like element IS1A family transposase	-
HZR24_RS23575	143308..143490	+	183	WP_000968309.1	hypothetical protein	-
-	143629..143690	-	62	NuclAT_0	-	-
-	143629..143690	-	62	NuclAT_0	-	-
-	143629..143690	-	62	NuclAT_0	-	-
-	143629..143690	-	62	NuclAT_0	-	-
HZR24_RS23580	143677..143883	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	-
HZR24_RS23585	144167..144424	+	258	WP_000083833.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / qnrS1 / dfrA14 / blaTEM-1B / sitABCD	iucA / iucB / iucC / iucD / iutA	1..154098	154098
	-	flank	IS/Tn	-	-	142510..143013	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15232.66 Da
• Isoelectric Point: 6.8536

>T167018 WP_001034044.1 NZ_CP058653:139193-139609 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T167018 NZ_CP074592:803386-803799 [Salmonella bongori serovar 40:z35:-]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTAATTTTATTGATGCCCTGGCCGTTGAGTTATACCCCATTGTGGATGATGCTACTGTCGCTGGTGGTGTTTGACTGCG
TTCGTAGTCAGCGCCGGATTAATACCTGTCAGGGAGAGATCAAGCTGCTAATGGATGGGCGCTTACGCTGGCAGGGGGAA
GACTGGGTGCTCCTGCGCCCGCCCTGGTTACTGAACAGCGGGATGGTGTTGCGTTTACGCGCCGAGTCCGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAGGCGGAATGGCGTGAGTTACGCCGTGTCATGTTGCAACAACCACTAT
CGGGCCAACACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8747.86 Da
• Isoelectric Point: 4.3775

>AT167018 WP_001261286.1 NZ_CP058653:138966-139196 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT167018 NZ_CP074592:803139-803405 [Salmonella bongori serovar 40:z35:-]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGCGACGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTGATGAATCACGGTAAACCGGCTGACGCTGAGCTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829CHW1

Antitoxin

• AlphaFold DB: A0A829CKZ6