Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 137881..138406 Replicon plasmid p1_035123
Accession NZ_CP058653
Organism Escherichia coli strain WCHEC035123

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HZR24_RS23540 Protein ID WP_001159871.1
Coordinates 137881..138186 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag HZR24_RS23545 Protein ID WP_000813630.1
Coordinates 138188..138406 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZR24_RS23525 133843..135009 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HZR24_RS23530 135597..136352 - 756 WP_000852146.1 replication initiation protein RepE -
HZR24_RS23535 137074..137880 - 807 WP_000016970.1 site-specific integrase -
HZR24_RS23540 137881..138186 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HZR24_RS23545 138188..138406 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HZR24_RS23550 138966..139196 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HZR24_RS23555 139193..139609 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
HZR24_RS23560 139684..141249 + 1566 WP_001128474.1 AAA family ATPase -
HZR24_RS23565 141234..142256 + 1023 WP_000361402.1 helicase UvrD -
HZR24_RS23570 142510..143207 - 698 Protein_155 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / qnrS1 / dfrA14 / blaTEM-1B / sitABCD iucA / iucB / iucC / iucD / iutA 1..154098 154098
- flank IS/Tn - - 142510..143013 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T167017 WP_001159871.1 NZ_CP058653:c138186-137881 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T167017 NZ_CP074588:768688-768957 [Pseudoalteromonas piscicida]
ATGAGTAGTAGTCAACGTTTACTGTCGTGGACTGATGACGCTTGGGATGACTATCTGTATTGGCAAACTCAAGATAAGAA
AACACTCAAGCGCATCAACAAACTCATCAATGATGTTAAGCGCTCCCCATTTGAGGGCATCGGTAAACCAGAGCCGTTGA
AAGAGAGTTTATCTGGTTTTTGGTCTCGTCGTATTGATGACACTAACAGGCTTGTTTATGCAGTTGATGATCAAGCGATA
ACGATAATTTCGTGTCGTTACCACTACTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT167017 WP_000813630.1 NZ_CP058653:c138406-138188 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT167017 NZ_CP074588:768441-768695 [Pseudoalteromonas piscicida]
ATGAGAATTGTATCTTTTACTGAAGCTAGAAATAGTCTTAAAGCTGTTCTAGACGGTGTAGTTAATGACGCAGATACAAC
AGTCATTACACGTCGTGACTCTGAGGATGCTGTGGTTATGTCTTTAGATTACTACAATAGCCTTATGGAGACTGTCCACT
TACTACGCTCTCCTCAAAACGCTGAACACTTAAATCGTTCGATTGCACAGTACCGTGCTGGTAAAACAACAGCACGAGAG
TTAATTGATGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References