Detailed information of TA system TA166986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 2776651..2778356
• Replicon: chromosome
• Accession: NZ_CP058644
• Organism: Pseudomonas yamanorum strain GZD14026

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HZF02_RS12585
• Protein ID: WP_179450115.1
• Coordinates: 2777481..2778356 (-)
• Length: 292 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: HZF02_RS12580
• Protein ID: WP_179450113.1
• Coordinates: 2776651..2777484 (-)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZF02_RS12560	2771856..2773352	-	1497	WP_179450106.1	aldehyde dehydrogenase	-
HZF02_RS12565	2773363..2774166	-	804	WP_179450107.1	aspartate dehydrogenase	-
HZF02_RS12570	2774177..2775859	-	1683	WP_179450109.1	thiamine pyrophosphate-binding protein	-
HZF02_RS12575	2775852..2776649	-	798	WP_179450111.1	SDR family oxidoreductase	-
HZF02_RS12580	2776651..2777484	-	834	WP_179450113.1	IclR family transcriptional regulator	Antitoxin
HZF02_RS12585	2777481..2778356	-	876	WP_179450115.1	alpha/beta hydrolase	Toxin
HZF02_RS12590	2778361..2778900	-	540	WP_155582117.1	cupin domain-containing protein	-
HZF02_RS12595	2778914..2779681	-	768	WP_179450117.1	SDR family oxidoreductase	-
HZF02_RS12600	2779678..2780454	-	777	WP_179450119.1	ABC transporter permease	-
HZF02_RS12605	2780483..2781475	-	993	WP_179450121.1	ABC transporter substrate-binding protein	-
HZF02_RS12610	2781516..2782367	-	852	WP_179450123.1	ABC transporter ATP-binding protein	-
HZF02_RS12615	2782814..2783212	+	399	WP_179450125.1	cytidine deaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (45-275)

Antitoxin

• Transcription regulator IclR, C-terminal (134-256)

Sequences

Toxin

Protein

• Length: 292 a.a.
• Molecular weight: 31147.74 Da
• Isoelectric Point: 7.4864

>T166986 WP_179450115.1 NZ_CP058644:c2778356-2777481 [Pseudomonas yamanorum]
MLPSVNHLPLTADLEARLVADFPQHTLKIDGGRQALRECGSGPAIVLLHGIGSGAASWLQVAQQLAPGARVIAWDAPGYG
DSTPLEDSAPKAAAYAQRLLQLLDGLHIEHCVLVGHSLGALTAVAFARLYANRVSRLVLISPARGYGDPARSQQRQEVRT
KRLRSLEQVGIQGNAEQRSAHMVSAKASPQALAWVRWNMARLNPDGYRQAIELLCDDDLLRHTPLSMPCEVHCGQDDGIT
PPEGCLPLAKALGTHLSLIPDAGHASPIEQPLVVAGRLAFAIKESLTGRTL

Nucleotide

• Length: 876 bp

>T166986 NZ_CP074578:c1859124-1858840 [Pseudomonas syringae]
ATGCAGGTTGAGTGGCTCAAAACAGCACTAAAGAATCTGGATGAGGAGGCAGCGTATATCGCACTGGACAATCCAGCTGC
TGCCGCTGCTTTCGTGAAAGCCATTCAATCAAACGTAACGCAGCTCGCGAGCTTTCCCGCCATGGGCCGGGAAGGACGGA
TTGCCGGAACCAGAGAATGGCCTCTGCCAGACCTGCCTTACCTGATTCCATATCGCATCCGTAGCGGCCGACTACAGGTT
CTACGGATTTTCCACACCAGAAGACAATCGCCACCCGTGTGGTAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30801.31 Da
• Isoelectric Point: 6.9639

>AT166986 WP_179450113.1 NZ_CP058644:c2777484-2776651 [Pseudomonas yamanorum]
MNDSDLLKDAQDKYIVPGLERGLLLLCEFTRRNRTLTAPELARRLALPRSTIFRLLTTLETMGFVTRSGNEYRLGMSVLR
LGFEYLASLELTELGQPLLARLCDRLNYPSNLVVRDGRSIVYVAKVSPPSVFSSAVNVGTRLPAHATVLGRILLEDLSLT
ELRELYPEEHLEQHSACTPKTVMELFDMVQADRQRGFVSGEGFFESAISTVAAPVRDQSGRIAAALGVTIPTTQIGHVNF
QELLSQVRRSADELSRLLNYNGAAQDGQHRVTALMRD

Nucleotide

• Length: 834 bp

>AT166986 NZ_CP074578:c1859353-1859114 [Pseudomonas syringae]
ATGTCCACAATGTCTCTACGTCTGCCGGACGAAATGGCAGATACCCTTGCACACCTTGCAAAGGCGACCGGGCGCAGCAA
GTCATTTTTGGCCCTCGACGCGCTCCGCGAATACCTGACACGCGAGGCTTGGCAGATTGCCGAAATTCAGCGTGCCATCG
AAGAAGCCGACGCCGGCGAGTTCGCAAGCCCAGACGACGTAAAAGCTGTCATGGACAAATGGTCAGGCAATGCAGGTTGA