Detailed information of TA system TA166981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 3634522..3635150
• Replicon: chromosome
• Accession: NZ_CP058638
• Organism: Rhodococcus sp. ZPP

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HYG77_RS17250
• Protein ID: WP_208960013.1
• Coordinates: 3634740..3635150 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HYG77_RS17245
• Protein ID: WP_208960012.1
• Coordinates: 3634522..3634743 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYG77_RS17220 (HYG77_17190)	3629651..3630451	+	801	WP_208960010.1	SDR family oxidoreductase	-
HYG77_RS17225 (HYG77_17195)	3631051..3631452	-	402	WP_109334518.1	hypothetical protein	-
HYG77_RS17230 (HYG77_17200)	3631623..3632819	+	1197	WP_244372918.1	histidine kinase	-
HYG77_RS17235 (HYG77_17205)	3632816..3633490	+	675	WP_230990003.1	response regulator transcription factor	-
HYG77_RS17240 (HYG77_17210)	3633682..3633876	+	195	WP_208960011.1	hypothetical protein	-
HYG77_RS40685	3634089..3634217	+	129	WP_280123267.1	hypothetical protein	-
HYG77_RS17245 (HYG77_17215)	3634522..3634743	+	222	WP_208960012.1	ribbon-helix-helix domain-containing protein	Antitoxin
HYG77_RS17250 (HYG77_17220)	3634740..3635150	+	411	WP_208960013.1	PIN domain nuclease	Toxin
HYG77_RS17255 (HYG77_17225)	3635383..3635859	+	477	WP_208960014.1	Lrp/AsnC family transcriptional regulator	-
HYG77_RS17260 (HYG77_17230)	3636006..3637205	+	1200	WP_244372920.1	GAF domain-containing protein	-
HYG77_RS17265 (HYG77_17235)	3637184..3637444	-	261	WP_208960015.1	DUF3263 domain-containing protein	-
HYG77_RS17270 (HYG77_17240)	3637441..3638460	-	1020	WP_208960016.1	lipoyl synthase	-
HYG77_RS17275 (HYG77_17245)	3638524..3639933	-	1410	WP_109334508.1	dihydrolipoyl dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-124)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-40)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14959.18 Da
• Isoelectric Point: 7.3247

>T166981 WP_208960013.1 NZ_CP058638:3634740-3635150 [Rhodococcus sp. ZPP]
MSGSTWLIDKSAMVRLGTSPDAEQWAKRIQRGLVHICTVTLLEVGCSARTAHDLSRGRRTPPLSAMPLEYSTPAVEDRAL
DVQRQLAERGQHRAPSIPDLLIAATAEINNRVVLHLDKDFDLIAAITSQPLERLVA

Nucleotide

• Length: 411 bp

>T166981 NZ_CP074577:61034-61450 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8405.45 Da
• Isoelectric Point: 4.7045

>AT166981 WP_208960012.1 NZ_CP058638:3634522-3634743 [Rhodococcus sp. ZPP]
MPDILIRNFPAEDLALLDEHAARLGISRTEFLRRQLQQEARRTATSVSTADLMRFSQDFPDLNDNEVMRGAWS

Nucleotide

• Length: 222 bp

>AT166981 NZ_CP074577:60807-61037 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA