Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 42145..42746 Replicon plasmid unnamed1
Accession NZ_CP058619
Organism Escherichia coli strain JS316

Toxin (Protein)


Gene name doc Uniprot ID A0A5M4JUN5
Locus tag HZF20_RS22970 Protein ID WP_001216041.1
Coordinates 42366..42746 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag HZF20_RS22965 Protein ID WP_001190712.1
Coordinates 42145..42366 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZF20_RS22905 37314..37670 + 357 WP_000403785.1 hypothetical protein -
HZF20_RS22910 37764..37946 + 183 WP_001224662.1 hypothetical protein -
HZF20_RS22915 37939..38115 + 177 WP_000753058.1 hypothetical protein -
HZF20_RS22920 38112..38471 + 360 WP_089477108.1 hypothetical protein -
HZF20_RS22925 38474..38965 + 492 WP_089477109.1 hypothetical protein -
HZF20_RS22930 38967..39092 + 126 Protein_61 hypothetical protein -
HZF20_RS22935 39408..39824 + 417 Protein_62 hypothetical protein -
HZF20_RS22940 39831..40697 + 867 Protein_63 hypothetical protein -
HZF20_RS22945 40681..40965 + 285 WP_001142396.1 hypothetical protein -
HZF20_RS22950 40950..41159 - 210 WP_001344848.1 hypothetical protein -
HZF20_RS22955 41333..41584 + 252 WP_032220968.1 DNA polymerase III subunit theta -
HZF20_RS22960 41683..42072 + 390 WP_073714448.1 DNA repair protein -
HZF20_RS22965 42145..42366 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HZF20_RS22970 42366..42746 + 381 WP_001216041.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HZF20_RS22975 42830..43630 - 801 WP_089477111.1 hypothetical protein -
HZF20_RS22980 43637..44338 - 702 WP_089477112.1 DUF2829 domain-containing protein -
HZF20_RS22985 44353..44847 - 495 WP_089477113.1 dUTP diphosphatase -
HZF20_RS22990 44844..45320 - 477 WP_001557738.1 hypothetical protein -
HZF20_RS22995 45317..45625 - 309 WP_000349257.1 hypothetical protein -
HZF20_RS23000 45735..46763 - 1029 WP_089477114.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..91318 91318


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13635.52 Da        Isoelectric Point: 5.6406

>T166960 WP_001216041.1 NZ_CP058619:42366-42746 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Download         Length: 381 bp

>T166960 NZ_CP074576:532396-532803 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGCGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT166960 WP_001190712.1 NZ_CP058619:42145-42366 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT166960 NZ_CP074576:532149-532415 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5M4JUN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References